Fig. 5
From: PECAM-1 drives β-catenin-mediated EndMT via internalization in colon cancer with diabetes mellitus
PECAM-1 defection and internalization enhanced the nuclear localization of β-catenin. a, b Localizations of PECAM-1 and β-catenin were observed after PECAM-1 siRNA treatment by immunofluorescence method under HG and SNs conditions, respectively. White arrows show the nuclear β-catenin; Yellow arrow heads show β-catenin in the membrane; White arrow heads show the colocation of PECAM-1 and β-catenin around the nucleus. c, d Total and phosphorylated β-catenin (Ser33) (a type to induce its degradation) were evaluated after PECAM-1 siRNA treatment by western blotting under HG and SNs conditions, respectively. The samples derive from the same experiment and that blots were processed in parallel. Data are means ± S.E.M. and analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. **p < 0.01, ****p < 0.0001