Fig. 2
From: PECAM-1 drives β-catenin-mediated EndMT via internalization in colon cancer with diabetes mellitus
HG and co-culture system induced the EndMT occurrence in vivo and in vitro. a Vimentin (arrow) and PECAM-1 (arrow head) were tested in endothelium of CC with and without DM by immunofluorescence. b, c PECAM-1 (arrow) and snail (arrow head) were analyzed in HG and SW480-HUVEC co-culture system. d, e EndMT markers were detected under HG and SNs conditions by western blotting. The samples derive from the same experiment and that blots were processed in parallel. NG: normal glucose (5.5 mmol/L), HG: high glucose (33 mmol/L), co-Cul: co-culture, SN-1: supernatant from SW620, SN-2: supernatant from HCT116. Data are means ± S.E.M. and analyzed using unpaired student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001