Fig. 4

Suppression of Hax1 alters the subcellular location of IQGAP1 at FAs. A, Representative immunofluorescence images of mock or Hax1 knockdown MCF7 cells stained for Vinculin (green) and IQGAP1 (red). Cells were imaged by super-resolution N-SIM microscopy. The boxed areas are magnified as insets. White arrows indicate accumulation of IQGAP1 at the leading edge in control cells. Red arrows point at IQGAP1 localization at vinculin-positive FAs in control cells. Scale bar, 10 μm. B, Co-localization of IQGAP1 and FA (vinculin) was determined by Pearson correlation coefficient (n = 20 cells for each). Note that IQGAP1 co-localization with vinculin at the rim of the leading edge is reduced significantly in Hax1-depleted cells (p < 0.0001). C, Presence of IQGAP1 and Hax1 in isolated FAs or WCL (20 μg) from mock or Hax1 KD cells was determined by immunoblots. D, Protein abundance in isolated FAs or WCL (20 μg) from cells treated with or without nocodazole was determined by immunoblot with different antibodies as indicated