Fig. 3

HCAR1-deficient mice exhibit anxiety-like behavior. Elevated plus maze test for anxiety-like behavior: a Representative images showing subject mouse movement in open and closed arms of the maze. b-d Quantitative behavioral measures for anxiety-like behaviors. HCAR1 KO mice traveled less distance (b), spent less time (c) and rested less time (d) in the open arm of the maze compared to close arm, indicating KO mice have more anxiety than WT. Data are mean ± s.d. from n = 16 for KO animal and n = 10 for WT animal. Analysis of Variance (ANOVA) was followed by Bonferroni post hoc correction test with * P < 0.05, ** P < 0.01 significance levels