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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Extracellular vesicle-associated tyrosine kinase-like orphan receptors ROR1 and ROR2 promote breast cancer progression

Fig. 2

ROR1 and ROR2 are enriched on tumor-derived lEVs and sEVs. A NTA of the three different EV subpopulations harvested from MCF-7 wildtype cells. B TEM of the MCF-7 EV subpopulations. The image on the left displays a wide-field overview of the sample. The panel on the right contains a close-up of the area marked with a black box in the wide-field image. C + D Western blot: Characterization of EVs isolated from (C) MCF-7 and (D) MDA-MB-231 wildtype cells for common EV markers. GM130 is shown as a negative marker. Equal amounts of protein were loaded in every lane. E Western blot: ROR1 or ROR2 expression on EVs harvested from MCF-7 cells transfected with ROR1/2-overexpression vectors (pROR1/pROR2) or respective empty vectors (pCMV/pcDNA). CD81 served as sEV marker, RGAP1 as marker for LOs and lEVs. Equal amounts of protein were loaded in every lane

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Cell Communication and Signaling

ISSN: 1478-811X