Fig. 7
P2Y2R knockout suppresses remodelling of the mouse femoral artery following injury. A(a) and A(b), H&E staining showing a normal femoral artery. A(c) and A(d), H&E staining showing a thickened intima and obvious neointimal lesions 3 weeks after guidewire injury of the femoral artery. A(e)–A(g), Statistical summary showing the media area, neointima area and ratio of neointima area to media area. Summary data were obtained from 33 slices of 11 uninjured control mice and 36 slices of 12 injured mice. B(a)–B(c), Immunofluorescence images and statistical summary of femoral artery Sects. 3 weeks post-injury showing a significant increase in Sca-1+ cell number. Statistical summary data obtained from 42 samples of 9 uninjured mice and 43 samples of 9 injured mice. C(a)-C(g), H&E staining showing that P2Y2R knockout reduces the changes in neointima area and ratio of neointima area to media area following femoral artery injury. Summary data were obtained from 42 slices of 14 control mice and 18 slices of 6 P2Y2R knockout mice. D(a)–D(c), Immunofluorescence images and statistical summary showing that P2Y2R knockout partly reverses the rise in Sca-1+ cell/total cell ratio following injury. Summary data were obtained from 43 slices of 9 control mice and 44 slices of 6 P2Y2R knockout mice. Nonparametric test (Mann–Whitney test) was performed for all above statistical tests. A P < 0.05 is considered a statistically significant test, and statistically significant P-values between the two groups are shown on the graph. Red, Sca-1+ cells expressing TdTomato; Green, Markers of mature smooth muscles (SMHHC); Blue, Hoechst33342-stained nuclei. Scale bar in the H&E staining figures represents 20 µm