Fig. 5
ATP-induced cell migration is mediated predominantly by the P2Y2R–ERK1/2 signalling pathway. A and B, Western blots showing that ATP (30 µM) activates ERK1/2 via Thr202/Tyr204 phosphorylation after 5 and 10 min of treatment. The histogram shows the relative activation level of ERK1/2 (pERK/ERK). C and D, ATP-activated ERK1/2 (Thr202/Tyr204 phosphorylation) is suppressed by the MEK inhibitor PD98059 (10 µM). E and F, ATP-activated migration is also suppressed by PD98059 (10 µM). G and H, P2Y2R-shRNA suppresses the phosphorylation of pERK1/2 induced by 30 μM ATP. Each statistic data in B, D, F were obtained from 3 independent experiments, while statistic data in H were obtained from 4 independent experiments. Data are expressed as mean ± standard deviation (SD). Data in B, D, F, and H were first tested by Shapiro–Wilk test for normality. Ordinary one-way ANOVA (Dunnett’s multiple comparisons test) was conducted for B, Ordinary one-way ANOVA (Tukey’s multiple comparisons test) was for F and unpaired t test was for D and H. A P < 0.05 is considered a statistically significant test, and statistically significant P-values between the two groups are shown on the graph