Fig. 4
ATP-induced [Ca2+]i elevations are mediated primarily by P2Y2Rs. A and B, The selective P2Y2R antagonist AR-C118925 significantly inhibits ATP-induced [Ca2+]i elevations. C and D, The selective P2Y6R antagonist MRS-2578 has a weaker effect, even at a high concentration (1 μM). E, Typical records showing that control shRNA transfection does not significantly alter ATP-induced [Ca2+]i elevations, while these [Ca2+]i elevations are suppressed by P2Y2R-shRNA transfection. F. Statistical results showing that P2Y2R-shRNA significantly inhibits [Ca2+]i elevations induced by 30 µM ATP. Summary data were obtained from 3 to 7 independent experiments. The ‘n’ with number in the graph is the number of cells measured under each treatment condition. Data are expressed as mean ± standard deviation (SD). Data were first tested by Shapiro–Wilk test for normality and then Ordinary one-way ANOVA (Tukey’s multiple comparisons test) was performed for B, D, and F. A P < 0.05 is considered a statistically significant test, and statistically significant P-values between the two groups are shown on the graph