Fig. 2

ATP promotes Sca-1 + cell proliferation through P2Y6R stimulation. A, Transcriptomic analysis revealing the main functional pathways involved in cell cycle changes induced by ATP. Molecular function (MF), cell component (CC) and biological process (BP). B, Effects of different ATP concentrations on the proliferation of Sca-1 + cells as measured by viable cell counting assay. C, The effects of ATP on proliferation are not markedly suppressed by the P2Y2R antagonist AR-C118925 (100 nM). D, Facilitation of proliferation by ATP is not blocked by specific P2Y2R knockout (D). E and F, Facilitation of proliferation by ATP is blocked by the P2Y6R antagonist MRS2578 (1 µM) (E) and by P2Y6R knockout (F). Summary data were obtained from 10–15 independent experiments. Data are expressed as mean ± standard deviation (SD). In B, C, and E figure, the indicated P-values were calculated using the Nonparametric test (Kruskal–Wallis test). In D and F figure, P-values were calculated using Mann–Whitney test and compared each group to the respective control. A P < 0.05 is considered a statistically significant test, and statistically significant P-values between the two groups are shown on the graph