Fig. 1
ATP promotes Sca-1+ stem cell migration through P2Y2R stimulation. A, Stimulation with ATP (0.3, 3, 30, 300 and 3000 µM) for 18 h dose-dependently promotes Sca-1+ cell migration in transwell assays. B, The non-specific P2R blocker suramin suppresses the facilitation of migration by ATP. C and D, The P2Y2R blocker AR-C118925 suppresses Sca-1+ cell migration induced by 30 μM ATP to a greater extent than the P2Y6R blocker MRS-2578. Images obtained at 100 × . E, A P2Y2R-shRNA also inhibits the migration of Sca-1+ cells induced by 30 μM ATP. Images obtained at 100 × . Summary data were obtained from 3–4 independent experiments. Data are expressed as mean ± standard deviation (SD). Data were first tested by Shapiro–Wilk test for normality. Ordinary one-way ANOVA (Tukey’s multiple comparisons test) was performed for A, C, D and E, while Brown-Forsythe and Welch One-way ANOVA (Dunnett’s multiple comparisons test) was performed for B. A P < 0.05 is considered a statistically significant test, and statistically significant P-values between the two groups are shown on the graph