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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: The B56γ3-containing protein phosphatase 2A attenuates p70S6K-mediated negative feedback loop to enhance AKT-facilitated epithelial-mesenchymal transition in colorectal cancer

Fig. 5

B56γ3 upregulates SNAIL and EMT hallmarks through AKT activity. A, B Lysates of HCT116 cells or C, D lysates of SW480 cells with the control vector (pMSCV or shLuc), stable overexpression of B56γ3 (4HAB56γ3), or stable knockdown of B56γ3 (shB56γ3) were analyzed by SDS-PAGE and Western blotting with specific antibodies as indicated. E Lysates of HCT116 or F lysates of SW480 cells with vector only (pMSCV) or stable overexpression of B56γ3 (4HAB56γ3) treated with DMSO or MK-2206(10 μM, Enzo Life Sciences) for 24 h were analyzed as described previously. G Lysates of HCT116 or H lysates of SW480 cells with vector only (pMSCV) or stable overexpression of B56γ3 (4HAB56γ3) transiently transfected with control siRNA or AKT1/2 siRNA (120 nM) were analyzed as described previously. The relative expression levels of SNAIL and E-cadherin were quantified by densitometry and normalized to that of β-actin. I HCT116 cells with vector only (pMSCV) or stable overexpression of B56γ3 (4HAB56γ3) transiently transfected with control siRNA or AKT1/2 siRNA (120 nM) were subjected to Transwell migration assay or J Transwell invasion assay as described earlier. The differences were assessed for statistical significance by Student’s t-test with p-values (* (< 0.05), ** (< 0.01), ***(< 0.001))

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Cell Communication and Signaling

ISSN: 1478-811X