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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: The B56γ3-containing protein phosphatase 2A attenuates p70S6K-mediated negative feedback loop to enhance AKT-facilitated epithelial-mesenchymal transition in colorectal cancer

Fig. 4

B56γ3 facilitates motility and invasion of CRC cells. A HCT116 cells B SW480 cells with control vector (pMSCV or shLuc), stable overexpression of B56γ3 (4HAB56γ3), or stable knockdown of B56γ3 (shB56γ3) were seeded onto the upper transwell chamber, and migrated cells were stained with crystal violet and counted 24 h after seeding. C HCT116 or D SW480 cells with the control vector (pMSCV or shLuc), stable overexpression of B56γ3 (4HAB56γ3), or stable knockdown of B56γ3 (shB56γ3) were seeded onto the upper chamber precoated with Matrigel for Transwell invasion analyses, and migrated cells were stained with crystal violet and counted 24 h after seeding. Representative images from at least three independent experiments are shown. Quantitation of migrated cells in different experimental groups was performed using ImageJ, and differences in relative cell numbers were assessed for statistical significance by Student’s t test with p value (*(< 0.05), **(< 0.01), ***(< 0.001)). (n = 3)

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Cell Communication and Signaling

ISSN: 1478-811X