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Table 2 Results of SV-AUC experiments for ggNesfatin-3 and hsNesfatin-3

From: Nesfatin-3 possesses divalent metal ion binding properties, which remain hidden in the nucleobindin-2 precursor protein

Protein

Agent

c (mg/ml)

rmsd

s20,w

f/f0

MMapp (kDa)

Oligomerization state

ggNesfatin-3

EDTA

(5 mM)

0.5

0.004678

1.53

2.17

2.12

26.1

43.8

Monomer (25%)

Dimer (75%)

0.75

0.005386

1.49

2.18

2.18

26.1

46.0

Monomer (24%)

Dimer (76%)

1.0

0.005703

1.53

2.19

2.26

28.5

49.0

Monomer (23%)

Dimer (77%)

Mg2+

(10 mM)

0.5

0.005294

2.28

1.89

39.7

Dimer (100%)

0.75

0.005872

2.00

2.40

1.92

33.4

43.9

Monomer (17%)

Dimer (83%)

1.0

0.006543

1.88

2.40

1.93

30.9

44.5

Monomer (8%)

Dimer (92%)

Ca2+

(10 mM)

0.5

0.004854

1.94

2.44

1.72

27.0

38.2

Monomer (26%)

Dimer (74%)

0.75

0.005517

1.91

2.50

1.80

28.2

42.4

Monomer (24%)

Dimer (76%)

1.0

0.006183

1.89

2.53

1.81

28.1

43.4

Monomer (23%)

Dimer (77%)

Zn2+

(100 µM)

0.5

0.005252

1.95

2.48

1.70

26.9

38.7

Monomer (26%)

Dimer (74%)

0.75

0.006271

1.82

2.45

1.78

26.0

41.0

Monomer (20%)

Dimer (80%)

1.0

0.005493

1.70

2.41

1.85

24.8

42.0

Monomer (21%)

Dimer (79%)

Zn2+

(300 µM)

0.5

0.005293

2.54

3.08

1.58

35.5

47.4

Monomer (41%)

Dimer (59%)

0.75

0.005616

2.50

3.06

1.59

35.4

47.9

Monomer (36%)

Dimer (64%)

1.0

0.006104

2.37

2.91

1.62

33.6

45.6

Monomer (30%)

Dimer (70%)

Zn2+

(800 µM)

0.5

0.005559

2.46

3.49

1.52

32.1

54.2

Monomer (10%)

Dimer (90%)

0.75

0.006844

2.46

3.49

1.52

32.0

54.3

Monomer (9%)

Dimer (91%)

1.0

0.006807

2.50

3.35

1.52

33.2

53.7

Monomer (12%)

Dimer (88%)

hsNesfatin-3

EDTA

(5 mM)

0.5

0.005253

2.44

2.19

56.1

Dimer (100%)

0.75

0.005695

2.42

2.28

58.6

Dimer (100%)

1.0

0.006596

2.41

2.38

62.2

Dimer (100%)

Mg2+

(10 mM)

0.5

0.005388

2.55

2.16

59.0

Dimer (100%)

0.75

0.005716

2.55

2.16

58.6

Dimer (100%)

1.0

0.006644

2.55

2.23

61.7

Dimer (100%)

Ca2+

(10 mM)

0.5

0.005404

2.32

2.80

1.84

40.1

53.4

Monomer (13%)

Dimer (87%)

0.75

0.005823

2.25

2.80

1.89

39.8

55.1

Monomer (9%)

Dimer (91%)

1.0

0.008080

2.07

2.76

1.93

36.3

55.6

Monomer (4%)

Dimer (96%)

Zn2+

(100 µM)

0.5

0.007259

3.14

1.47

45.1

Dimer (100%)

0.75

0.008686

3.19

1.54

49.4

Dimer (100%)

1.0

0.009081

3.03

4.93

1.64

50.1

104.0

Dimer (98%)

Tetramer (2%)

Zn2+

(300 µM)

0.5

0.007857

3.65

1.38

51.4

Dimer (100%)

0.75

0.007742

2.90

3.61

1.41

37.7

52.2

Monomer (11%)

Dimer (89%)

1.0

0.010962

3.50

1.41

49.6

Dimer (100%)

Zn2+

(800 µM)

0.5

0.007036

3.94

1.31

53.6

Dimer (100%)

0.75

0.007492

3.93

5.78

1.36

55.8

99.6

Dimer (99%)

Tetramer (1%)

1.0

0.008561

3.92

5.73

1.36

55.0

97.0

Dimer (99%)

Tetramer (1%)

  1. Numbers in brackets indicate the percentage of each fraction and were determined by considering 100% for the sum of the main indicated types of sedimenting species. rmsd – root-mean-square deviation; s20,w – sedimentation coefficient under standard conditions (i.e., water, 20 °C); f/f0 – frictional ratio (the ratio of the actual frictional coefficient to that for an anhydrous sphere with an equal volume); MMapp – apparent molecular weight derived from SV-AUC experiments
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Cell Communication and Signaling

ISSN: 1478-811X