Table 1 Secondary structure composition of ggNesfatin-3 and hsNesfatin-3 in the presence of various metal ions. The deconvolution of CD data was performed using CDNN [20]. The table presents the percentage of each structural element in the secondary structure. The data are the average of three independent experiments
Protein | Agent | Concentration (mM) | α-helix (%) | Antiparallel β-sheet (%) | Parallel β-sheet (%) | β-turn (%) | Random coil (%) |
|---|---|---|---|---|---|---|---|
ggNesfatin-3 | EDTA | 5 | 32 ± 1.3 | 8 ± 0.3 | 9 ± 0.4 | 17 ± 0.2 | 33 ± 1.0 |
MgCl2 | 10.00 | 38 ± 1.0 | 7 ± 0.2 | 8 ± 0.2 | 16 ± 0.1 | 31 ± 0.6 | |
0.010 | 35 ± 0.6 | 8 ± 0.1 | 9 ± 0.2 | 16 ± 0.1 | 32 ± 0.5 | ||
0.025 | 34 ± 0.1 | 8 ± 0.0 | 9 ± 0.1 | 17 ± 0.0 | 32 ± 0.1 | ||
0.050 | 38 ± 1.0 | 7 ± 0.2 | 8 ± 0.2 | 16 ± 0.2 | 30 ± 0.6 | ||
CaCl2 | 0.075 | 39 ± 1.5 | 7 ± 0.2 | 8 ± 0.3 | 16 ± 0.2 | 30 ± 1.0 | |
0.100 | 40 ± 0.4 | 7 ± 0.0 | 8 ± 0.1 | 16 ± 0.1 | 29 ± 0.2 | ||
0.250 | 44 ± 2.3 | 6 ± 0.3 | 7 ± 0.5 | 16 ± 0.3 | 27 ± 1.4 | ||
0.500 | 44 ± 0.2 | 6 ± 0.0 | 7 ± 0.1 | 16 ± 0.0 | 27 ± 0.1 | ||
0.750 | 44 ± 0.0 | 6 ± 0.2 | 7 ± 0.2 | 16 ± 0.2 | 26 ± 0.8 | ||
1.000 | 44 ± 0.5 | 6 ± 0.1 | 7 ± 0.1 | 15 ± 0.1 | 28 ± 0.3 | ||
10.00 | 45 ± 3.0 | 6 ± 0.5 | 7 ± 0.6 | 15 ± 0.4 | 27 ± 1.7 | ||
0.050 | 45 ± 1.1 | 6 ± 0.2 | 7 ± 0.2 | 15 ± 0.2 | 27 ± 0.6 | ||
0.100 | 45 ± 0.5 | 6 ± 0.1 | 7 ± 0.1 | 15 ± 0.1 | 27 ± 0.3 | ||
ZnCl2 | 0.200 | 47 ± 1.0 | 6 ± 0.1 | 7 ± 0.2 | 15 ± 0.1 | 25 ± 0.6 | |
0.300 | 46 ± 0.8 | 6 ± 0.1 | 7 ± 0.1 | 15 ± 0.1 | 26 ± 0.4 | ||
0.400 | 44 ± 1.6 | 6 ± 0.2 | 7 ± 0.3 | 15 ± 0.2 | 26 ± 1.0 | ||
0.500 | 43 ± 0.4 | 6 ± 0.1 | 7 ± 0.1 | 15 ± 0.1 | 27 ± 0.2 | ||
0.600 | 41 ± 0.6 | 7 ± 0.1 | 7 ± 0.1 | 16 ± 0.1 | 29 ± 0.4 | ||
0.800 | 33 ± 0.7 | 8 ± 0.2 | 9 ± 0.2 | 17 ± 0.1 | 33 ± 0.5 | ||
hsNesfatin-3 | EDTA | 5 | 32 ± 1.3 | 14 ± 0.8 | 8 ± 0.2 | 18 ± 0.1 | 28 ± 0.3 |
MgCl2 | 10.00 | 38 ± 2.7 | 10 ± 0.8 | 8 ± 0.4 | 18 ± 0.1 | 26 ± 1.4 | |
0.010 | 39 ± 0.7 | 11 ± 0.3 | 7 ± 0.1 | 18 ± 0.0 | 25 ± 0.2 | ||
CaCl2 | 0.025 | 42 ± 0.9 | 9 ± 0.3 | 7 ± 0.1 | 18 ± 0.1 | 24 ± 0.3 | |
0.050 | 48 ± 0.2 | 7 ± 0.1 | 6 ± 0.1 | 17 ± 0.1 | 22 ± 0.1 | ||
0.075 | 49 ± 1.3 | 7 ± 0.4 | 6 ± 0.2 | 17 ± 0.1 | 21 ± 0.6 | ||
0.100 | 49 ± 2.7 | 7 ± 0.8 | 6 ± 0.3 | 17 ± 0.4 | 21 ± 1.1 | ||
0.250 | 49 ± 0.9 | 7 ± 0.4 | 6 ± 0.1 | 17 ± 0.4 | 21 ± 0.3 | ||
0.500 | 49 ± 2.5 | 7 ± 0.7 | 6 ± 0.4 | 17 ± 0.4 | 21 ± 1.0 | ||
0.750 | 52 ± 3.7 | 6 ± 1.1 | 6 ± 0.6 | 17 ± 0.6 | 20 ± 1.5 | ||
1.000 | 50 ± 1.9 | 6 ± 0.5 | 6 ± 0.3 | 17 ± 0.3 | 21 ± 0.9 | ||
10.00 | 51 ± 0.6 | 6 ± 0.1 | 6 ± 0.1 | 17 ± 0.1 | 21 ± 0.3 | ||
0.050 | 43 ± 0.3 | 8 ± 0.1 | 7 ± 0.1 | 17 ± 0.0 | 25 ± 0.1 | ||
0.100 | 44 ± 0.1 | 7 ± 0.1 | 7 ± 0.1 | 17 ± 0.0 | 25 ± 0.2 | ||
0.200 | 45 ± 1.6 | 7 ± 0.5 | 7 ± 0.3 | 17 ± 0.3 | 25 ± 0.7 | ||
ZnCl2 | 0.300 | 44 ± 0.2 | 7 ± 0.1 | 7 ± 0.0 | 17 ± 0.0 | 26 ± 0.1 | |
0.400 | 45 ± 1.5 | 6 ± 0.4 | 7 ± 0.3 | 16 ± 0.2 | 26 ± 0.6 | ||
0.500 | 43 ± 1.2 | 7 ± 0.3 | 7 ± 0.2 | 16 ± 0.1 | 27 ± 0.6 | ||
0.600 | 44 ± 1.9 | 7 ± 0.6 | 7 ± 0.3 | 16 ± 0.3 | 26 ± 0.9 | ||
0.800 | 43 ± 1.4 | 7 ± 0.3 | 7 ± 0.2 | 16 ± 0.1 | 27 ± 0.7 |