Fig. 5From: Nesfatin-3 possesses divalent metal ion binding properties, which remain hidden in the nucleobindin-2 precursor proteinIntrinsic fluorescence spectra of Nucb2s in the presence of 5 mM EDTA, 10 mM Mg2+, and 10 mM Ca2+ and in the presence of increasing concentrations of Zn2+ (100–800 µM). Panels show the fluorescence spectra of ggNesfatin-3 (A–B) and hsNesfatin-3 (C–D). For clarity, only selected curves are shown (A, C). The protein concentration was 3 µM. The measurements were performed at 20 °CBack to article page