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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Nesfatin-3 possesses divalent metal ion binding properties, which remain hidden in the nucleobindin-2 precursor protein

Fig. 4

SDS‒PAGE analysis of limited proteolysis products at different Mg2+ (A, D), Ca2+ (B, E), and Zn2+ (C, F) concentrations (expressed in mM) indicated on the top of each panel. Both ggNesfatin-3 (A–C) and hsNesfatin-3 (D–F) were incubated with V8 protease at 20 °C at an enzyme-to-substrate ratio of 1:5000. MW – molecular mass marker; CTRL – control reactions with no protease

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Cell Communication and Signaling

ISSN: 1478-811X