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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Nesfatin-3 possesses divalent metal ion binding properties, which remain hidden in the nucleobindin-2 precursor protein

Fig. 3

SDS‒PAGE analysis of limited proteolysis products at a range of digestion times (expressed in minutes) indicated on the top of each panel (up to 240 min for ggNesfatin-3 and 24 h for hsNesfatin-3). Both ggNesfatin-3 (A–E) and hsNesfatin-3 (F–J) were incubated with V8 protease at 20 °C and at an enzyme-to-substrate ratio of 1:5000 in the absence (A, F) and presence of 10 mM MgCl2 (B, G), 10 mM CaCl2 (C, H), 0.1 mM ZnCl2 (D, I) and 0.8 mM ZnCl2 (E, J). MW – molecular mass marker; -V8 – control reactions with no protease

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Cell Communication and Signaling

ISSN: 1478-811X