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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Carbonic anhydrase IX inhibitor S4 triggers release of DAMPs related to immunogenic cell death in glioma cells via endoplasmic reticulum stress pathway

Fig. 1

S4 decreases glioma cell viability. A HA, Hs683, LN229, and U87MG cells were vehicle-treated or treated with varying concentrations of S4 (0.01,0.1,1, 10, 100 μM) for 24, 48, 72 h respectively. Cell growth was determined using the CCK8 assay. IC 50 values were listed in the margins of each column. B Cells (Hs683, LN229, and U87MG) were vehicle-treated or treated with 60 μM S4 and cultured in complete media for 14 days for colony formation analysis. C LN229 and U87MG cells cultured in 3D medium were treated with DMSO or 60 μM S4 for 12 days and examined for the spheroid formation. (Scale bar = 100 μm). D LN229 and U87MG cells were treated with DMSO or 60 μM S4, then assessed by immunofluorescence staining with ki67 or DAPI. (Scale bar = 100 μm). The above experiments were performed three times (*P < 0.05, **P < 0.01, ***P < 0.001)

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Cell Communication and Signaling

ISSN: 1478-811X