Table 3 The oligo sequencing for sgRNA cloning used in the study
gRNA oligonucleotides | ||
|---|---|---|
sgRNAs | gRNA oligos | Sequence (5ʹ-3ʹ) |
NONO gRNA1 | F | CACCGCAATCCGTTCGACGACGACG |
R | AAACCGTCGTCGTCGAACGGATTGC | |
NONO gRNA2 | F | CACCGCCTAGCGGAGATTGCCAAAG |
R | AAACCTTTGGCAATCTCCGCTAGGC | |
Control gRNA | F | CACCGAAACGGCGGATTGACCGTAA |
R | AAACTTACGGTCAATCCGCCGTTTC | |