Table 2 List of primers used for Real-Time PCR
Gene | Forward primer sequence | Reverse primer sequence |
|---|---|---|
NONO | 5ʹ- GGAGCCCATGGACCAGTTAG-3ʹ | 5ʹ- AAATCTGGGTGGCTGCTCTC-3ʹ |
PIN1 | 5ʹ- TTTGAAGACGCCTCGTTTGC-3ʹ | 5ʹ-GTGCGGAGGATGATGTGGAT-3ʹ |
p21 | 5ʹ-CTGCCCAAGCTCTACCTTCC-3ʹ | 5ʹ-CGAGGCACAAAGGGTACAAGA-3ʹ |
GAPDH | 5ʹ- GTGAACCATGAGAAGTATGACAAC -3ʹ | 5ʹ- CATGAGTCCTTCCACGATACC -3ʹ |
OCT4 | 5ʹ-GAGAACCGAGTGAGAGGCAAC-3ʹ | 5ʹ- CTGATCTGCTGCAGTGTGGGT-3ʹ |
SOX2 | 5ʹ-TTTGTCGGAGACGGAGAAGC-3ʹ | 5ʹ-TAACTGTCCATGCGCTGGTT -3ʹ |
Slug | 5ʹ- ACGCCTCCAAAAAGCCAAAC -3ʹ | 5ʹ- ACTCACTCGCCCCAAAGATG -3ʹ |
Twist | 5ʹ-CTCGGACAAGCTGAGCAAGA-3ʹ | 5ʹ-GCTCTGGAGGACCTGGTAGA-3ʹ |
Cyclin E1 | 5ʹ-ATACTTGCTGCTTCGGCCTT-3ʹ | 5ʹ- TCAGTTTTGAGCTCCCCGTC-3ʹ |