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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/β-catenin pathways in human breast cancer cells

Fig. 8

NONO depletion inhibits the cancer stem cell formation in breast cancer cells. a FACS measured the subpopulation of cells with CD24−/CD44+ phenotype in MDA-MB-231 and MCF-7 cells. The bar graph shows the quantification of CD24−/CD44+ cells. b, c qRT-PCR expression analysis of stemness-associated markers, including SLUG, OCT4, SOX2, Twist in MDA-MB-231 and MCF-7 cells. Data presented as mean standard deviation ± SD of three independent experiments. d, e Quantification NONO- and PIN1 expression in NONO-depleted MDA-MB-231 and MCF-7 cells were analyzed by qPCR. f Western blotting analysis of NONO and PIN1 protein in NONO and PIN1-depleted MDA-MB-231 and MCf-7 cells. β-actin was used as a control for equal loading and ImageJ software for densitometry analysis. a-e Data represented as mean standard deviation ± SD of three independent experiments. ***p < 0.001, p** < 0.01, *p < 0.05

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Cell Communication and Signaling

ISSN: 1478-811X