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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/β-catenin pathways in human breast cancer cells

Fig. 6

Silencing of NONO induces apoptosis in MDA-MB-231 and MCF-7 cells. a PI and Annexin V staining of NONO-depleted MDA-MB-231, and MCF-7 cells were determined by flow cytometry after 72 h of transfection. The percentage of apoptotic cells was plotted for MDA-MB-231 and MCF-7 cells. **p < 0.01. b, c Expression of Bcl-2 and Bax in MDA-MB-231 and MCF-7 and procaspase 3 and cleaved caspase 3 in MCF-7 were analyzed by Western blot analysis. d Expression of the apoptotic proteins p53 and p-p53 in NONO-depleted MCF 7 cells. Treatment with 50 µM H2O2 for 6 h promotes NONO expression, while treatment with the p53 inhibitor PIF-α for 24 h does not affect NONO expression. e The effect of NONO silencing on mitochondrial membrane potential alteration (MMP) by JC-1. a, e Data presented as mean ± SD of three independent experiments. **p < 0.01. b, c, d Representative blots from at least two independent experiments are shown. β-actin was used as an internal control

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Cell Communication and Signaling

ISSN: 1478-811X