Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/β-catenin pathways in human breast cancer cells

Fig. 5

Silencing of NONO gene expression induces S-phase cell cycle arrest in MDA-MB-231 and MCF-7 breast cancer cells. a silencing of NONO induced accumulation of cells in the S phase as shown by flow cytometry. b Quantification of cell cycle-regulating genes using qRT-PCR in MDA-MB-231 and MCF-7 cells. NONO silencing affects the expression of cell cycle-related genes. CCE1 (CyclinE) was significantly downregulated while CDKN1A (P21) was strongly upregulated in MDA-MB-231 and MCF-7 breast cancer cells. a, b Data presented as mean standard deviation ± SD of three independent experiments. p* < 0.05, **p < 0.01, ***p < 0.001. c Western blot analysis of PCNA in control and NONO-silenced MDA-MB-231 and MCF-7 cells. PCNA expression was inhibited in both cell lines. β-actin was used as an internal control, ImageJ software for densitometry analysis

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X