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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/β-catenin pathways in human breast cancer cells

Fig. 4

Silencing of NONO inhibits the tumorigenesis and colony formation ability of MDA-MD-231 and MCF-7 breast cancer cells. a, b siRNA-NONO is efficient in knocking down the NONO gene at the transcriptional level and the corresponding protein level, as shown by RT-PCR and Western blot, respectively. c Crystal violet assay demonstrated the proliferative ability of breast cancer cells transfected with siRNA-NONO and siRNA-scr. d The clonogenicity of NONO-silenced breast cancer cells was determined by colony formation assay. The bars show the mean standard deviation ± SD of at least triplicate replicates. **p < 0.01, ***p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X