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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/β-catenin pathways in human breast cancer cells

Fig. 3

The stability of NONO protein is enhanced by PIN1. a MDA-MB-231 cells were treated with juglone or DMSO. About 48 h later, the cells were treated with MG132 for 8 h. Western blot analysis revealed the involvement of PIN1 in NONO protein abundance and regulation via the proteasomal inhibition pathway. β-actin was used as an internal control and ImageJ software for densitometry analysis. b Representative IHC image of PIN1 protein expression in breast tumors and normal tissue. Scale bar, 50 µm. c The distribution of PIN1-IHC signals in breast and normal tissue was analyzed using the quick score method. The unpaired Mann-Whitney test was used to compare IHC quick scores in tumor tissue with versus normal. d A significant association (Spearman’s rank correlation coefficient r = 0.464, p = 0.01, n = 20) between NONO and PIN1 proteins was observed in breast cancer. p* < 0.05, ***p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X