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Fig. 10 | Cell Communication and Signaling

Fig. 10

From: Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/β-catenin pathways in human breast cancer cells

Fig. 10

NONO activates MAPK/β-catenin signaling pathway in breast cancer cells. a Total cellular protein (50 µg) from siRNA-transfected MDA-MB-231 and MCF-7 cells was analyzed by Western blotting with Akt/MAPK antibodies to the proteins as indicated. b, c The interaction of NONO and c-Jun was examined by yeast two-hybrid and immunoprecipitation/Western blotting. d The immunoprecipitated complex of MDA-MB-231 cell lysates with anti-NONO or anti-β-catenin antibody analyzed by Western blotting (IB). e Silencing of NONO in MDA-MB-231 and MCF-7 cells suppresses β-catenin and related downstream molecules such as c-myc and cyclin D1. a, e Representative blots from two independent experiments are shown. β-actin was used as a control for equal loading and ImageJ software for densitometry analysis

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Cell Communication and Signaling

ISSN: 1478-811X