Fig. 6

Inhibition of Cx43-GJs function attenuated Ang II-increased [Ca²⁺]_i, F-actin polymerization and MLC2 phosphorylation. A Detection of [Ca²⁺]_i with fluo3-AM by flow cytometry on HUASMCs. B F-actin was stained with FITC-phalloidin (green) and cell nuclei was stained with DAPI (blue) in HUASMCs. Each dot represents the fluorescence intensity of one field. Three different fields were captured in every experiment. The experiment was repeated 3 times (n = 3 per group). C p-MLC2 and MLC2 were labeled by cellular immunofluorescence (red) and cell nuclei were stained by DAPI (blue) in HUASMCs. Each dot represents the fluorescence intensity of one field. Three different fields were captured in every experiment. The experiment was repeated 3–4 times (n = 3–4 per group). D Quantitation of fluorescence intensity (B, C) in each group. *p < 0.05, ** p < 0.01, *** p < 0.001. All values expressed as mean ± SEM