Fig. 4

Enhancing Cx43-GJs function with retinoic acid reversed propofol-induced excessive relaxation in Ang II-pretreated HUASMCs. A F-actin was stained with FITC-phalloidin (green) and cell nuclei was stained with DAPI (blue) in HUASMCs. B p-MLC2 and MLC2 were labeled by cellular immunofluorescence (red) and cell nuclei were stained by DAPI (blue) in HUASMCs. C Bar plots showed the statistical results of F-actin level, p-MLC2 level and MLC2 level. Each dot represents the fluorescence intensity of one field. Three different fields were captured in every experiment. The experiment was repeated 3–5 times (n = 3–5 per group). ** p < 0.01, *** p < 0.001. All values expressed as mean ± SEM