Fig. 2
Propofol induced a greater magnitude of [Ca2+]i decline in Ang II-pretreated HUASMCs. A Detection of [Ca2+]i with fluo3-AM by flow cytometry on HUASMCs. B The change rate of [Ca2+]i in mean between Control vs. Control + propofol and Ang II vs. Ang II + propofol. C F-actin was stained with FITC-phalloidin (green) and cell nuclei was stained with DAPI (blue) in HUASMCs. Each dot represents the fluorescence intensity of one field. Three different fields were captured in every experiment. The experiment was repeated 4 times (n = 4 per group). D p-MLC2 and MLC2 were labeled by cellular immunofluorescence (red) and cell nuclei were stained by DAPI (blue) in HUASMCs. Each dot represents the fluorescence intensity of one field. Three different fields were captured in every experiment. The experiment was repeated 4 times (n = 4 per group). *p < 0.05, ** p < 0.01, *** p < 0.001. All values expressed as mean ± SEM