Fig. 1

Propofol induced excessive relaxation in Ang II-pretreated HUASMCs compared to normal HUASMCs. A F-actin was stained by FITC-phalloidin (green), p-MLC2 and MLC2 were labeled by cellular immunofluorescence (red) and cell nuclei were stained by DAPI (blue) in HUASMCs. Each dot represents the fluorescence intensity of one field. Three different fields were captured in every experiment. The experiment was repeated 3–5 times (n = 3–5 per group). B F-actin was stained with FITC-phalloidin (green) and cell nuclei was stained with DAPI (blue) in HUASMCs. Each dot represents the fluorescence intensity of one field. Three different fields were captured in every experiment. The experiment was repeated 3 times (n = 3 per group). C p-MLC2 and MLC2 were labeled by cellular immunofluorescence (red) and cell nuclei were stained by DAPI (blue) in HUASMCs. Each dot represents the fluorescence intensity of one field. Three different fields were captured in every experiment. The experiment was repeated 3–5 times (n = 3–5 per group). D The change rate of F-actin and p-MLC2/MLC2 in mean between Control vs. Control + propofol and Ang II vs. Ang II + propofol. *p < 0.05, ** p < 0.01, *** p < 0.001. All values expressed as mean ± SEM