Fig. 7
Macrophage PTEN deficiency‑mediated STING enhances stress‑induced hepatocyte necroptosis via modulating RIPK3 signaling activation. Bone marrow-derived macrophages (BMMs) were isolated from PTENM−KO mice and then transfected with CRISPR/Cas9-mediated STING ACT and control vector. A ELISA analysis of supernatant TNF-α levels with or without LPS-stimulated BMMs (n = 6 samples/group). B The figure for macrophage/hepatocyte co-culture system. BMMs transfected with CRISPR/Cas9-mediated ACT were stimulated with LPS 6 h and then cocultured with primary hepatocytes supplemented with H2O2 for 24 h. C ELISA analysis of LDH release from hepatocytes in cocultures (n = 6 samples/group). D Western-blot analysis and relative density ratio of RIPK3 and p-MLKL in hepatocytes after coculture (n = 6 samples/group). E Immunofluorescence staining and fluorescence intensity analysis of p-MLKL+ hepatocytes after co-culture (n = 6 samples/group). Scale bars, 30 μm. All data represent the mean ± SD. *p < 0.05. **p < 0.01, ***p < 0.001