Fig. 2
Macrophage PTEN deficiency inhibits STING-mediated TBK1 activation and diminishes APAP-induced hepatocyte death. PTENFL/FL and PTENM−KO mice were subjected to APAP (400 mg/kg) challenge for 24 h. A Western-assisted analysis and relative density ratio of PTEN, p-STING, STING, p-TBK1, and TBK1 in the PTENFL/FL livers with or without APAP-treated (n = 6 samples/group). B Immunofluorescence staining of PTEN and CD68 in AILI livers (n = 6 mice/group). Scale bars, 100 μm. C Western-assisted analysis and relative density ratio of p-STING, STING, p-TBK1, TBK1, p-IRF3, IRF3, p-P65, P65, RIPK3, and p-MLKL in the PTENFL/FL and PTENM−KO mice livers after APAP challenged (n = 6 samples/group). D Immunohistochemistry staining of RIPK3 expression in APAP-challenged livers (n = 6 mice/ group). Scale bars, 200 and 40 μm. E Quantitative RT-PCR-assisted detection of Tnf-α, IL-1β, IL-6, Cxcl-1, and Mcp-1 mRNA levels in PTENFL/FL and PTENM−KO mouse livers with or without APAP exposure (n = 6 samples/group). All data represent the mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001