Fig. 1
Macrophage PTEN deficiency alleviates APAP-induced liver injury and decreases macrophage/neutrophil infiltration. Mice were subjected to APAP (400 mg/kg) challenge for 24 h. A Western blot assay detected the PTEN expression in hepatocytes and liver macrophages from PTENFL/FL and PTENM−KO. Representative of three experiments. B Representative histological staining (H&E) of APAP-conditioning liver tissue and Suzuki’s histological score (n = 6 samples/group). Scale bars, 200 μm. C Animal survival curves following a solitary APAP dose administered over 72 h (n = 6 mice/group). D Hepatocellular function, assessed by serum ALT (sALT) and AST (sALT) levels (IU/L) (n = 6 samples/group). E Immunofluorescence staining of F4/80 macrophages in injury livers (n = 6 mice/group). Quantification of F4/80+ macrophages. Scale bars, 100 μm and 30 μm. F Immunohistochemistry staining of Ly6G neutrophils in injury livers (n = 6 mice/group). Quantification of Ly6G+ neutrophils. Scale bars, 100 and 30 μm. All data represent the mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001