Fig. 6From: P38-DAPK1 axis regulated LC3-associated phagocytosis (LAP) of microglia in an in vitro subarachnoid hemorrhage modelBECN1 contributing to P38-DAPK1-regulated LAP. The mRNA expression of Dapk1, Becn1 and Rubicon after transfection of DAPK1-specific plasmid (a). Immunofluorescence showing the co-location of DAPK1 and BECN1, DAPK1 and RUBICON (b). The change of co-location coefficient between DAPK1 and BECN1 (c), DAPK1 and RUBICON (d) after oxyHb exposure. Ubiquitylation of BECN1 after oxyHb exposure (e) and SB203580 (SB) added (f). ****-P < 0.0001, ***-P < 0.001, *-P < 0.05; ns, non-significant. Scale bar: 5 μmBack to article page