Fig. 6

Effects of MrgD on Gα_i in NRCM and NRCF. A-B, MrgD onerexpression inhibited Ang II-induced mRNA expression levels of Gα_i1, Gα_i2 and Gα_i3 in NRCM and NRCF. C-D, The decreases of Gα_i1, Gα_i2 and Gα_i3 induced by Ang II in NRCM and NRCF were reversed by MrgD knockdown. E, PTX pretreatment blocked the protective effects of MrgD shRNA on Ang II-induced fetal genes expression. F, Ad-MrgD has no extra effects on enhancing Ang II-induced the changes of fetal genes. G, PTX pretreatment blocked the protective effects of MrgD shRNA on Ang II-induced the increases of the α-SMA, TGF-β and collagen I in NRCF. H, Ad-MrgD has no extra effects on enhancing Ang II-induced the changes of the α-SMA, TGF-β and collagen I in NRCF. The data are expressed as mean ± SEM. n = 3 biological replicates per group. ^nsP > 0.05, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001