Fig. 5

Effects of Ala on MrgD expression in NRCM and NRCF. A-B, MrgD was detected in the membrane, cytoplasm, and nuclear region of NRCM, and the number of NRCM with positive MrgD was increased in Ala or Ang II groups. C, Ala or Ang II increased the mRNA level of MrgD in NRCM. D, Ala or Ang II treatment increased the MrgD protein level in NRCM. E, Ala inhibited the increase in MrgD level in the nuclei of NRCM. F-G, MrgD overexpression increased the MrgD levels in the total protein or nuclei of NRCM. H-I, MrgD was detected in the membrane, cytoplasm, and nuclear region of NRCF, and the number of NRCF with positive MrgD was increased in Ala or Ang II groups. J, Ala or Ang II increased the mRNA level of MrgD in NRCF. K, Ala or Ang II treatment increased the MrgD level in total protein of NRCF. L, Ala inhibited the increase in MrgD level in the nuclei of NRCF. M–N, MrgD overexpression increased the MrgD protein level or that in nuclei of NRCF. The data are expressed as mean ± SEM. n = 4, 5 or 6 biological replicates per group. ^nsP > 0.05, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001