Fig. 3

Effects of MrgD KO on cardiac hypertrophy and fibrosis. A-C, Cardiac size, HW/BW and HW/TL were decreased after MrgD KO in the heart compared with those in the Ang II + MrgD WT group. D, the mRNA levels of Nppa, and Nppb, as well as the ratio of Myh 6/7 in the hearts from different groups. E, MrgD KO decreased the cardiac fibrosis level in the Ang II-induced rats, as indicated by masson and picrosirius red staining. F-G, the protein and mRNA levels of α-SMA, TGF-β and collagen I in the hearts from different groups. The results are expressed as mean ± SEM. n = 6 biological replicates per group. ^nsP > 0.05, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001