Fig.3From: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signalingMyofiber specific TGF-β-Smad2/3 signaling effects on macrophages efferocytosis in inflamed muscle. Tunel staining of apoptotic cells (A). Immunofluorescence results of macrophages-associated apoptotic cells (F4/80+Tunel+) and free apoptotic cells (F4/80−Tunel+) (B). FACS analysis of the proportion of all apoptotic cells (Tunel+) (C), free apoptotic cells (Annexin-V+ Tunel+) (D) and efferocytotic macrophages (Annexin-V−F4/80+Tunel+) (E). Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD (n = 3). (*P < 0.05). Bar = 50 μmBack to article page