Fig. 1

The inflammatory stimulus drives the myofiber specific TGF-β signal activation. A, RNA-seq analysis showing the mRNA level changes of TGF-β2 and TGF-βr2 of inflamed TA muscle. B, The mRNA levels of TGF-β2 and TGF-βr2 of inflamed muscle were quantified by qRT-PCR. C, Western blot analysis of protein levels in TGF-β2, p-TGF-βr2 and p-Smad2/3 in inflamed muscle. The relative protein level values were expressed as a ratio (protein of interest/GAPDH or phosphorylated (p) protein/total protein). D, Immunofluorescence double-staining results of TGF-βr2, p-TGF-βr2, p-Smad2/3 and Dystrophin in inflamed muscle. E, Western blot analysis of the protein levels of TGF-β2, p-TGF-βr2 and p-Smad2/3 in MPC-Myotubes receiving pro-inflammatory administration. Statistical data were expressed as the mean ± SD (n = 3). Multiple comparisons were analyzed by One-way ANOVA (*P < 0.05, **P < 0.01). Bar = 50 μm