Fig. 8

Old APN KO mice shows the most highly retarded EL-4 tumor growth via increase in infiltration of BMSCs and CD8⁺ T cells. A The protein levels of APN in both bone marrow (BM) and white adipose tissue (WAT) at indicated months were measured by western blotting. B The population of young and old APN KO BMSCs (Sca-1⁺CD44⁺CD11b⁻CD45⁻) cultured under 1% hypoxia was analyzed by flow cytometry, and bar graphs were used for quantitative data. C BMSCs were placed in the upper chamber of the transwell plate and migrated cells were imaged under light microscopy (× 100 magnification) after 24 h, and then images were quantified using ImageJ software. D The mRNA level of HIF1α in BMSCs from young and old APN KO mice was measured by real-time PCR E EL-4 tumors were excised from young and old APN KO mice 20 days after administration, and tumor volume was measured. F The population of infiltrated BMSCs (Sca-1⁺CD44⁺CD11b⁻CD45⁻) in EL-4 was analyzed by flow cytometry. G The population of infiltrated CD8⁺ T cells (CD3⁺CD8⁺) in EL-4 cells was analyzed by flow cytometry. H The mRNA level of Ccl8 in young and old APN KO mice was measured by real-time PCR. All data were expressed as the mean ± SD from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001