Fig. 6

BMSCs within EL-4 tumor stimulates the infiltration of CD8⁺ T cells. A APN KO BMSCs and EL-4 cells were placed in the upper and lower chambers of the transwell plate, respectively. Migrated cells were imaged under light microscopy (× 100 magnification) after 24 h. B The mRNA level of Has3 in EL-4 cells was measured by real-time PCR after treatment at the indicated concentration of 4-Mu for 24 h. C APN KO BMSCs were placed in the upper chamber of the transwell plate, and EL-4 was placed in the bottom chamber of the transwell plate with or without 4-Mu (2 mM), and migrated cells were imaged under light microscopy (× 100 magnification) after 24 h. D EL-4 tumors were excised from WT and APN KO mice 21 days after administration, and tumor volume was measured. E The population of infiltrated MSCs was analyzed by flow cytometry. All images were quantified using ImageJ software, and bar graphs were used for quantitative data. F, G Representative immunofluorescence IHC staining images were used to detect the colocalization of HIF1α and CD44 from EL-4 tumor sections and shown as a quantitative bar graph. Scale bar = 300 μm. All data were expressed as the mean ± SD from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001