Fig. 4

APN-induced GSK3β inactivation inhibits STAT3/HIFα axis. A Protein levels of pGSK3β (S9) and GSK3β were analyzed by western blotting. B APN KO BMSCs were cultured in the presence or absence of rAPN (10 μg/ml), and the protein levels of pGSK3β (S9) and GSK3β were analyzed by western blotting. C APN KO BMSCs were pretreated with SB216763 (10 μM) for 3 h followed by the treatment of rAPN (10 μg/ml) for 24 h, and the protein level of HIF1α was analyzed by western blotting. D The protein levels of pAKT (S473), and AKT in APN KO BMSCs were analyzed by western blotting. All images were quantified using ImageJ software, and bar graphs were used for quantitative data representation. All data were expressed as the mean ± SD from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001