Fig. 3

HIF1α stimulates SCF expression. A The mRNA levels of Tnf-α, Scf, Il-1β, Ifn-γ, Il-10, and Il-6 were measured by real-time PCR in BM and BMSCs. B Levels of c-kit were analyzed using flow cytometry, and bar graphs were used for quantitative data. C APN KO BMSCs were cultured in the presence or absence of DCC-2618 (2 ng/ml), and the protein levels of STAT3, pSTAT3 (Y705), and HIF1α were analyzed by western blotting, and bar graphs were used for quantitative data. D APN KO BMSCs were cultured in the presence or absence of vitexin (20 μM), and the mRNA level of Scf was measured by real-time PCR. N.S stands for not significant. All data were expressed as the mean ± SD from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001