Fig. 5
From: Physiological and pathological consequences of exosomes at the blood–brain-barrier interface
Monitoring the effect of dementia with Lewy bodies patient Exos on intracellular accumulation of phosphorylated proteins. Detection of exosomal α-synuclein using immuno-gold labeling and TEM images (Ctl: Control; DLB: dementia with Lewy bodies patient) A. Stereotaxic injection of prepared Exos into C57BL/6 N × DBA/2 F1 mouse hippocampus B. Bright-field imaging of brain slides after injection of Exos C [row 1: Sagittal view of the hippocampus. The site of needle injection is highlighted by arrowheads (Scale bar: 150 μm). Arrows indicate immuno-labeled cell bodies (Scale bar: 25 μm). Brain samples were stained using anti- phosphorylated α-synuclein (pSer129), PHF1 and Aβ (6E10) [(Scale bar: 25 μm]. Semi-quantification of immunohistochemical stains using an optical density. *p < 0.05. Reprinted with permission [85], Copyright 2017. Acta Neuropathologica Communications