Fig. 2From: Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancerDifferentiation capacity of clones derived from NAT, HLT and tumor tissues. A Immunofluorescence images of ALI mini-guts stained with E-cadherin (green) and Muc2 (red). Nuclei (blue) were stained with DAPI. Scale bar, 50 μm. B Immunofluorescence images of ALI mini-guts stained with KRT20 (green) and CHGA (red). Nuclei (blue) were stained with DAPI. Scale bar, 50 μm. C Immunofluorescence images of ALI mini-guts stained with KRT20 (green) and Villin (red). Nuclei (blue) were stained with DAPI. Scale bar, 50 μm. D Heatmap shows normalized gene expression of stem cell clones (Clone) on feeder cells and corresponding ALI mini-guts (ALI). E Gene expression of stemness markers in stem cells clones and corresponding ALI mini-guts based on RNA-seq dataBack to article page