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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Colonic stem cells from normal tissues adjacent to tumor drive inflammation and fibrosis in colorectal cancer

Fig. 1

Establishment of clones derived from NAT, HLT and tumor tissues. A Overview of experimental design. The clones were derived from NAT and paired CRC tissues, as well as clones from HLT. B Representative H&E staining images of the original tissues. Scale bar, 50 µm. C Representative bright field microscopy images of clones from NAT, HLT and CRC tissues on lawns of irradiated 3T3-J2 feeder cells. Scale bar, 100 µm. D Rhodamine staining showing the clonogenicity assay of colonies grown on feeder cells following seeding 1,000 single cells per well from each clone derived from NAT, HLT and CRC tissues. E Histogram of clonogenicity index based on percentage of plated cells that form colonies. Data represented as mean ± SD; n = 3 technical replicates

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Cell Communication and Signaling

ISSN: 1478-811X