Fig. 5

Tyro3 deficiency decreases cytokine expression in joint tissue of mice bearing arthritis. The knockout mice lacking Tyro3 and their littermate control received K/BxN serum injection. On day 12 the mice were euthanized, followed by removal of the ankle joints. RNA was purified from joint tissue and cytokine mRNA levels were measured by quantitative RT-PCR. The results were normalized against mRNA of β-actin and relative levels of quantification were calculated. Data are means ± SEM, n = 6. For comparison of the effect of gene deficiency, two way ANOVA with the Tukey’s post hoc correction analyses was used. *, p < 0.05; **, p < 0.01