Fig. 5

OMV-mediated RNase1 mRNA repression is independent of JAK/STAT and NF-κB signaling. HULEC-5a were pretreated for 1 h with 5 µM JAKi (Ruxolitinib) or NF-κBi (BAY-11–7082) followed by 16 h stimulation with SEC-purified OMVs from Ec, Kp and Sal (MOV₁₀₀₀) as well as IFN-γ (250 ng/ml) or TNF-α (10 ng/ml) as indicated, left untreated as control (Ctrl) or treated with DMSO as solvent control (Ctrl-DMSO). mRNA expression of RNase1 (A and C), B) CXCL10 or D) ICAM-1 was determined by qPCR, normalized to RPS18 and the respective Ctrl. n = 3–6, line at mean, Two-way ANOVA with Tukey‘s multiple comparison test. * compared to corresponding Ctrl, # as indicated. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ^#p < 0.05, ^##p < 0.01, ^####p < 0.0001