Fig. 2

OMVs repress endothelial RNase1 and induce proinflammatory EC activation. Human lung microvascular ECs (HULEC-5a) were stimulated for 16 h and 24 h with SEC-purified OMVs (MOV₁₀₀₀) from Ec, Kp and Sal or TNF-α (10 ng/ml) or left untreated as control (Ctrl). mRNA expression of A) RNase1 C) CXCL8, E) ICAM-1 and F) CXCL10 was determined by qPCR, normalized to RPS18 and untreated cells (Ctrl). B) RNase1 and D) CXCL8 protein release in supernatants of 16 h stimulated HULEC-5a was measured by ELISA, depicted as x-fold protein relative to control (Ctrl). n = 3–4, line at mean, One-way ANOVA with Dunnett‘s multiple comparison test compared to respective Ctrl. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001