Fig. 3
From: Tricyclic antidepressants induce liver inflammation by targeting NLRP3 inflammasome activation
Nortriptyline induces NLRP3-dependent ASC oligomerization by targeting mtROS accumulation but has a weak effect on potassium efflux and no effect on Ca2+ mobilization. a The ASC oligomerization from BMDMs stimulated with nortriptyline was evaluated by western blot. b Mobilization of Ca2+. c Detection of intracellular K+. d The mitochondrial damaged cells were determined via JC-1 staining. e Cells were treated with LPS and nortriptyline and then the mtROS content was evaluated by flow cytometry. f–i BMDMs were incubated with LPS and Mito-TEMPO followed by nortriptyline treatment, the content of mtROS (f) was evaluated by flow cytometry, the expression of caspase-1 in SN (g) was assessed via western blotting and the generation of IL-1β (h) or TNF-α (i) in SN was detected using ELISA. Data are presented as mean ± SEM (n = 3); *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant; One-Way ANOVA followed by Dunnett's post hoc test